A protocol for analysis of mammalian cells containing firefly and renilla luciferase genes, grown in multiwell plates. The powerplex y23 system provides all materials necessary to amplify ystr regions of human genomic dna, including a hotstart dna polymerase, and is compatible with the abi prism 3100 and 3100 avant genetic analyzers and applied biosystems 3, 3 xl, 3500 and 3500xl genetic analyzers. Quick 2prtc thermal cycling use of the powerplex y23 system to amplify dna from storage card punches nstructions for use of products dc2305 and dc2320. The powerplex fusion 6c system provides all materials necessary to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplex fusion 6c 5x master mix. The sv total rna isolation system provides a fast and simple technique for preparing purified and intact total rna from tissues, cultured cells and white blood cells in as little as one hour, depending on the number of samples to be processed. The powerplex cs7 allelic ladder mix was analyzed with an applied biosystems 3 genetic analyzer using a 3kv, 5second injection. The powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping forensic casework samples, database samples and paternity samples. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Navigate to the panels text file downloaded in the getting started section. The file is checked using the validator software and issues, e. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability.
Jul 12, 2012 promega corporation announces the newest addition to the powerplex str systems family, the powerplex y23 systema rapid human identification yshort tandem repeat ystr assay for forensic casework, offender databasing and relationship testing. These products are intended for preparation of singlesource reference, database and paternity samples where sample purification is unnecessary. Id like to have a full download of the whole database or subdatabase. The powerplex fusion system was developed to amplify both extracted dna and dna from solid support materials such as fta cards, swabs, and nonfta punches without compromising performance. Either provide a file containing your haplotypes or enter your haplotype manually. Validation of powerplex y23 system promega using reduced. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block. Powerplex fusion 6c system amplification of 1 ng 2800m dna. Oefelein, qa managersenior dna analyst at dna labs international. New york genomeweb news promega said today that its powerplex y23 system has been approved for use in laboratories generating dna records for the national dna index system. The powerplex 6c matrix standard allow the powerplex fusion 6c system to be analyzed on the applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers cat. A concordance study, forensic science international.
The 5dye str multiplex amplifies 23 ystr loci, including two rapidly mutating ystr loci. However, they were all far from guizhou shui, which suggested that the former three ethnic groups were genetically different from guizhou shui. Like the wizard plus sv dna purification system cat. Richard iii is one of the most famous and controversial english kings. Jan 30, 2015 promega has released the powerplex fusion 6c system for forensic casework samples. It combines common and informative loci to overcome typical challenges faced by laboratories, including sample inhibitors, low levels of input dna, degradation, and mixtures, promega said. Genetics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Promega amplification protocol recommends a pcr total reaction volume of 25. In conclusion, the powerplex y23 system provided substantially strong discriminatory power in the shui population in guizhou. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Validation and implementation of the promega powerplex. Biallelic polymorphism of ag variation at position 308 in. Maleline relatives were typed using promega powerplex y23 and for snps defining the main.
Powerplex y23 system reproducibly amplifies trace template amounts as. This manual contains protocols for use of the powerplex fusion 6c system with the geneamp pcr system 9. Validation and implementation of the promega powerplex y23. Product components and storage conditions product size cat.
Use of the powerplexr y23 system to amplify extracted dna. Information about other promega fluorescent str systems is available upon request from promega or online at. Use of different str kits including powerplex esx 17, powerplex esi 17, and ampflstr ngm, which have different loci adjacent to d3s58, helped verify that the mystery allele was associated with a large d3s58 allele because apparent trialleles changed with each str kit result. The powerplex esx 17 system is compatible with the abi prism 310, 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers. Promega new powerplexr y23 str system reveals more ystr. Genetic profile of 23 y chromosomal str loci in guizhou shui. Shakespeare said, the evil that men do lives after them. This document is a quick protocol for experienced users to amplify extracted dna. The powerplex 16 system is a multiplex str system for use in dna typing, including paternity testing, forensic dna analysis, human identity testing and tissue culture strain identification. Yfiler, powerplex y23 promega powerplex y23, yfiler plus applied biosystems. The powerplex y23 system allows ystr analysis of human forensic and database samples. Powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping of database, paternity and forensic casework samples. The powerplex fusion 6c is a sixcolor, 27locus multiplex system. This standard ensures minimal risk of human dna contamination for products used to collect, store and analyze biological materials for forensic purposes.
The powerplex 6c matrix standard consists of dna fragments labeled with six different fluorescent dyes in one tube. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block with max mode as the ramp speed. The kit contains all 12 loci in powerplex y system, the additional 5 loci found in ampf l str yfiler, plus 6 new loci. The sample file was analyzed with the genemapper id software, version 3. Quick 2prtc thermal cycling use of the powerplex y23 system to amplify dna from swabs instructions for use of products dc2305 and dc2320. Identification of the remains of king richard iii nature. The powerplex y23 system allows ystr analysis of human forensic and. Visit the web site to verify that you are using the most current version of this technical manual. The reagent can be added directly to cells in growth medium without washing, and induces cell lysis and acts as a substrate for firefly luciferase, producing a luminescent signal that can be read over a period of two hours. Highly discriminatory capacity of the powerplex y23 system. Strengths and limitations of ngs for forensic dna analysis.
Developmental validation of the powerplex fusion system for. The powerplex fusion system allows coamplification and fluorescent detection of the core codis us. By using the function search using the export file all haplotypes in the file will be displayed as a list. Maxwell, plexor, powerplex and powerquant are registered trademarks of promega. The purpose of the present study is to validate the powerplex y23 system using half volume reaction mix for the amplification of a wide range of forensic samples. Apr 12, 2017 todays promega connections blog is written by guest blogger rachel h. This is continually true in the case of unidentified remains throughout the united stat.
The file needs to be prepared according to the example file found at the bottom of the search page. A fast, sensitive and reliable ystr multiplex system for forensic and population genetic purpose. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Reagent promega in the reaction mix as described in the technical manual. Developmental validation of the powerplex fusion 6c system. Importing powerplex y23 panels, bins and stutter text files into genemapper idx. Powerplex y23 promega corporation pp y23 system is a 5dye multiplex genotyping kit that analyzes 17 ystr loci, commonly available in other ystr multiplex kits along with that 6 new ystr.
Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Development and validation of a rapid pcr method for the. Promega forensic products are manufactured in alignment with the iso 18385 standard. Promega offers a range of products for cell biology and drug discovery, many of which are built upon bioluminescence technology. Wsp cld forensic dna continuing education day june 18, 2015. Use of the powerplex r y23 system to amplify dna from.
Promega was an early supplier in the cellfree protein synthesis field and is continuing to develop its portfolio in this area. Validation and implementation of the promega powerplex y23 str system marla kaplan dna technical leader. Promega powerplex y23 system receives ndis approval. This approach allows one system to be used for both casework and database activities. A decrease in the amplification time would allow for increased laboratory throughput, which may help reduce backlog when is due to limited cycling capacity. Str alleles and amplification artifacts pdf free download. The powerplex y23 system also is compatible with direct amplification, enabling streamlined ystr databasing efforts. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource. Features of powerplex y23 system showing the diverse utility of pp y23. Email promega technical services if you have questions on use of this system. Thermal cycling time is cut roughly in half, and the system detects substantially more ystr. Dualluciferase reporter assay system protocol promega.
The powerplex cs7 system is a multiplex str assay for relationship testing and human identification, allowing coamplification and threecolor detection of seven str loci, including lpl, fb, fesfps, fa01, penta d, penta c and penta e. By using the speedstar hs polymerase takara bio, otsu, japan in combination with the veriti applied biosystems, foster city. Amplification setup was performed either using the amplification volume recommended in the powerplex y23 technical manual pcr total. Power plex y 23 system markers source powerplex y23 system manual.
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